Application Note: Pathway-Targeted Metabolomic Analysis in Oral/Head and Neck Cancer Cells Using Ion Chromatography-Mass Spectrometry
7 August 2017
Metabolomics aims to measure a wide breadth of small molecules (metabolome) in the context of physiological stimuli or disease states. Ion chromatography (IC) is often employed in the separation of charged molecules and can be coupled with MS for targeted screening and quantification of metabolites in biological samples. In this application note, a high-flow-rate IC system was utilized for targeted analysis of the TCA cycle intermediates to shorten the run time and increase throughput and robustness in order to accommodate the hundreds of samples typically analyzed in a targeted metabolomics project.