MSIA Workflow for Therapeutic Antibodies: Qualitative, Quantitative, and Functional Verification Data from HR/AM Detection of Intact, Reduced, and Peptide-level Forms of Adalimumab
15 Aug 2016This application note illustrates an innovative and versatile MSIA Workflow approach to therapeutic antibody analytics, using adalimumab in human donor plasma as a model biological system and subsequent analysis by three distinct mass spectrometry detection formats: Intact, Reduced, and Bottom up. The biopharmaceutical industry has adopted biologics as a major component of their drug portfolios and developing pipelines. The rapid adoption of this class of biologics is due to their high affinity for their targets, selective biological actions, predictability of common side effects, and standardization of manufacturing processes.
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Thermo Scientific™ ProSwift™ IEX Protein Column
Thermo Fisher ScientificMonolith Ion-Exchange Columns for Superior High-Resolution Analytical and Preparative Protein Separations The ProSwift SCX monolith column line has been expanded to include a new 1 x 50 mm column. As with all ProSwift monoliths, the fast mass transfer allows for fast protein separations. ProSwift monoliths offer excellent separations using fast gradients at low and high flow rates, which improve productivity.ProSwift Monolith Ion Exchange columns are the column of choice for fast separations with high resolution for protein separation and purification, delivering higher resolution than porous bead-based columns while providing high capacity. ProSwift IEX monoliths provide the outstanding resolving power of nonporous analytical media combined with fast separation performance and exhibit reproducibility even after hundreds of runs.ProSwift® IEX Protein Column Features: High resolution High loading capacity Fast separations Wide range of operational flow rates Excellent stability over a wide pH range Outstanding reproducibility and ruggedness Optimal performance in a broad variety of applications High throughput and improved productivity Column Details: Chemistry: Tertiary amine, Quarternary amine, Caboxylic acid, Sulfonic acid Length: 50 mm ID: 1.0, 4.6 mm