ResourceGeneral Lab

Measuring dead volume in INTEGRA’s multichannel reagent reservoirs

25 Aug 2020

Dead volume is a real problem when transferring reagents into multiwell plates using a multichannel pipette, especially when handling expensive or rare solutions. To overcome this issue, INTEGRA developed a two-compartment reservoir that enables pipette tips to sit on the bottom and prevents liquids from pooling, offering the lowest dead volume on the market. In this technical note, INTEGRA provides experimental data to demonstrate this, and guidance to support users in setting up their experiments.

SureFlo™ Multichannel Reagent Reservoirs

INTEGRA Biosciences

INTEGRA reagent reservoirs consist of disposable inserts that fit into reusable bases with clearly visible volume markings. This cost- and space saving design has now been combined with the innovative SureFlo™ anti-sealing array. This unique technology enables pipette tips to sit on the bottom without sealing or creating a vacuum. Along with the hydrophilic surface treatment that prevents liquid from pooling, these reservoirs offer the lowest dead volumes on the market. The reagent reservoirs are – as the models without SureFlo™ array – available in sizes of 10, 25 and 100 ml. Features and benefits: •    SureFlo™ anti-sealing array prevents pipette tip seal off which can cause liquid to pop into the tip, filter or even the pipette itself •    Unique surface treatment spreads liquid evenly offering even lower dead volumes •    Reuse the sturdy base and toss the disposable insert  

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25 ml Divided Reservoir with Lowest Dead Volume

INTEGRA Biosciences

25 ml divided reservoirs are comprised of 5 and 10 ml compartments, while still nesting comfortably in the standard 25 ml reusable, sturdy reservoir base. Each reservoir features the SureFlo™ anti-sealing array and hydrophilic surface treatment providing especially low dead volumes. 

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