Application Note: LC-MRM Quantification of Src-Family Kinases using Protein Standards Expressed in Cell-Free Systems
7 July 2015

This poster describes a method for the quantification of SFK expression using peptides unique to each sequence. Each of the SFK proteins screened in this study produced at least 2 unique peptides, and all SFK proteins could be detected and quantified in a single experience when applied in parallel, offering a clear advantage over immunoblotting.