Application Note: KAPA2GTM Robust HotStart Mouse Genotyping
12 September 2014

This application note demonstrates the KAPA2G Robust HotStart DNA Polymerase. This product is a highly robust and versatile second-generation enzyme derived through a process of molecular evolution, that exhibits high performance across a range of crude sample PCR applications. With KAPA2G Robust HotStart, DNA fragments ≤1 kb may be amplified directly from crude tissue lysates, prepared from mouse tail or ear clippings in as little as 10 minutes. Combined with the faster extension rate of KAPA2G Robust HotStart, mouse genotyping workflows may be significantly streamlined and turnaround times reduced to 2 hours.