Application Note: Intact identification of adeno-associated virus capsid proteins
22 February 2023
In this application note, Phenomenex profiles denatured and dissociated
capsid proteins from AAV5 and AAV9, via analysis with LC-MS using
a BioZen™ Intact XB-C8 reversed phase column. The use of adeno-associated viruses (AAV) as an effective vector
for the delivery of ssDNA in gene therapy continues to gain
momentum. A chief attribute to this success is the broad tissue
tropism enabled by a range of serotypes. To date, approximately 13
different serotypes have been identified with a range of shared
amino acid sequence identity. Structurally, the viral capsid is a
sixty-protein icosahedron comprised of three capsid, or viral
proteins (VP), non-covalently assembled in a ratio of 1:1:10
(VP1:VP2:VP3).