Application Note: Improvements in sensitivity for quantification of steroid phosphate drugs using ACQUITY PREMIER and MaxPeak HPS Columns
19 February 2021

In this application note, Waters shows the impact of using a novel surface for quantifying hydrocortisone phosphate and dexamethasone phosphate in extracts of human plasma. Using the ACQUITY PREMIER System and MaxPeak HPS Columns, Waters were able to achieve a 10-fold improvement in the lowest limit of quantification (LLOQ) for hydrocortisone phosphate and 7.5-fold increase in LLOQ for dexamethasone phosphate.