Imaging-based analysis of cellular transfection efficiency

While transfection efficiency is usually assessed manually by fluorescence microscopy or using single-cell analysis techniques such as flow cytometry – which requires detachment of the cells from the plates – live cell imaging permits the analysis of the cell expression in situ. Tecan’s new Spark^® Cyto combines the multimode detection of a microplate reader with a sophisticated camera that enables bright field and fluorescencebased imaging of cell samples directly in the microplate well, controlled by the versatile and user-friendly SparkControl™ software.