Application Note: High Resolution Melt Parameter Considerations for Optimal Data Resolution
7 November 2013

The popularity of high resolution melt (HRM) analysis has grown considerably in the last few years and HRM analysis now encompasses applications including single nucleotide polymorphism (SNP) analysis, mutation scanning, DNA methylation analysis and species identification. This study analyses the steps performed by HRM software to identify thermal profile differences and examines the effect of assay optimization techniques.