Application Note: Gram Scale Antibody Production Using CHO Cell Transient Gene Expression (TGE) via Flow Electroporation
2 May 2014
This technical note presents data demonstrating the reproducibility, scalability, and antibody production capabilities of MaxCyte electroporation. Secreted antibody titers routinely exceed 400 mg/L and can exceed 1gram/L following optimization, thereby enabling multi-gram antibody production from a single, CHO cell transfection. Data is also presented showing the use of MaxCyte electroporation for the rapid generation of high-yield stable CHO cell lines to bridge the gap between early and late stage antibody development activities.