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Future Proofing the Biopharmaceutical QC Lab: Benefits of Automating Mobile Phase Delivery to Improve pH Consistency in Size Exclusion Chromatography Methods

28 Jan 2015

This application note in the series on transitioning HPLC-based biopharmaceutical separations to UPLC-based methods focuses on the benefits of automating mobile phase delivery. The study described demonstrates the benefits of Auto•Blend Plus Technology for consistent and reliable delivery of pH-dependent mobile phase for SEC-HPLC and SEC-UPLC.

ACQUITY UPLC H-Class PLUS Bio System

Waters

The ACQUITY UPLC H-Class PLUS Bio System delivers the benefits of UPLC's resolution, sensitivity, and throughput in a system purpose-built for the analysis of proteins, peptides, nucleic acids, and glycans.

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ACQUITY UPLC BEH Columns

Waters

In 2004, Waters revolutionized liquid chromatography with the introduction of the ACQUITY UPLC System. At the heart of the system was Waters second generation hybrid, the Ethylene Bridged Hybrid (BEH) particle. In 2005, BEH Technology preparative particle sizes (2.5, 3.5, 5 and 10 μm) with the introduction of XBridge between HPLC and UPLC Technology platforms. BEH Technology particles offer: Excellent peak shape and efficiency for basic analytes A rational array of chromatographic selectivity Improvements in chemical stability at mobile phase extremes, particularly at elevated pH

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Empower™ Chromatography Data System

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The Empower Chromatography Data System is Waters’ compliance-ready chromatography data software (CDS) for advanced data acquisition, processing, and reporting that simplifies the management of chromatography test results.

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Future Proofing the Biopharmaceutical QC Lab: Benefits of Automating Mobile Phase Delivery to Improve pH Consistency in Size Exclusion Chromatography Methods