Full Characterization of Heterogeneous Antibody Samples Under Denaturing and Native Conditions on the Q Exactive BioPharma Mass Spectrometer

  The Thermo Scientific™ Q Exactive™ Plus and Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometers adds superior denatured and native MS intact analysis and subunit top/middle-down analysis capabilities to the most powerful benchtop peptide mapping instruments available. When combined with Thermo Scientific™ BioPharma Finder™ software it provides a complete integrated hardware and software solution for BioPharma characterization.   The Thermo Scientific™ Q Exactive™ BioPharma platform offers distinct operational modes that have been optimized for the top 3 protein characterization workflows: High Mass Range (HMR) mode was designed specifically for intact therapeutic protein workflows with the aim of providing ease of use an flexibility as needed. HMR mode is optimized for both conventional intact monoclonal antibody analysis performed in denaturing conditions, as well as native MS analysis which is ideal for antibody drug conjugates (ADCs) and other heterogeneous antibody samples Protein mode on the Q Exactive HF is a powerful combination that has set the standard for the highest quality isotopically resolved data achievable on a benchtop mass spectrometer. Deconvolution of subunit MS spectra is easy and powerful with Thermo Scientific™ BioPharma Finder™ Software Peptide mapping with Standard mode is state-of-the-art with the Q Exactive BioPharma, which is a culmination of decades of leadership in the development of data dependent acquisition (DDA)