Fast Analysis of Isoflavones in Dietary Supplements – Method Transfer to an ACQUITY UPLC H-Class System

28 Feb 2019

In this application note, the efficacy of different methods for the determination of isoflavones in dietary supplements was determined. The standard methods are based on reversed-phase high-performance liquid chromatography (RP-HPLC) and ultraviolet and visible light (UV-VIs) spectroscopy detection. By transferring a new isoflavones standard method onto the ACQUITY Arc UHPLC System, the run time of the analysis was cut from 70 minutes to 18 minutes.

ACQUITY QDa Mass Detector

Waters

The ACQUITY QDa® Mass Detector gives you certainty above all else. You can minimize the risk of unexpected coelutions or components and confirms trace components with the analytical confidence of mass detection. The ACQUITY QDa® Detector is a mass detector built around the needs of analytical scientists for chromatographic analysis. Robust, reliable and requiring no sample adjustments, it just integrates with your current LC, ACQUITY UPLC, ACQUITY UPC2 and purification systems. You'll enhance the analytical value and productivity of each analysis, and you won't need to run all the additional assays or time-consuming alternative techniques or even wait for results from specialist labs that cost your laboratory productivity. With the ACQUITY QDa® Detector, you now have information-rich mass spectral data to complement data from your current Waters® optical detectors, including the ACQUITY UPLC PDA, TUV, ELS, and FLR detectors, as well as the ACQUITY UPC² PDA Detector. This mass spectral information integrates seamlessly into the same workflow; routinely giving you more complete separation characterization. Features: Intuitive operation More information from every sample Effortless integration Increased efficiency Resolving complexity Applications: Food & Environment Life Sciences and Pharmaceuticals Chemical

(20)

Links

Tags

Fast Analysis of Isoflavones in Dietary Supplements – Method Transfer to an ACQUITY UPLC H-Class System