Fast, Accurate and Reproducible Method for Simultaneous Quantitation of Pyridoxal 5’-phosphate and Pyridoxic Acid in Human Plasma by HPLC with Fluorescent Detection
17 Dec 2014Fluorescence detection has been used nearly exclusively by a majority of reference laboratories in HPLC analysis of vitamin B6 due to its selectivity and high sensitivity. However, the published derivatization procedures suffer from tedious sample preparation and short HPLC column lifetimes. This study aimed to simplify the derivatization and sample preparation steps when analyzing PLP and pyridoxic acid (PA) by HPLC-FLD.
Related Products
Request Quote for All Products
Gemini pH Flexible LC Columns
Phenomenex IncSetting the Standard for pH Method DevelopmentRugged reversed phase HPLC columns that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations. Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity Expect longer column lifetime with patented TWIN-NX™ technology Ideal for analytical and preparative separations of basic and acidic compounds Who needs Phenomenex's Gemini HPLC columns? Method Developers: Extreme pH method flexibility Reproducible performance Purification Chemists: Purify bases at high pH Increased loading Increased retention Increased resolution Lab Managers: Extended column lifetime Consistent results.