Application Note: Extraction of Vitamin D Metabolites From Human Plasma Using ISOLUTE® SLE+
2 August 2011

This application note from Biotage, describes a simple and robust procedure for the extraction of vitamin D metabolites from human plasma at clinically significant concentrations using ISOLUTE SLE+ supported liquid extraction plates. The latter is an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation time.