Extracting Genomic DNA from Plant Material with the sbeadex® Plant Kit from LGC Genomics

10 Dec 2014

Reliable, standardized and high quality DNA preparations, delivered by chemistry adapted robotic platforms are key elements of many genomics based plant breeding programs. The sbeadex® mini plant kit has been developed to extract genomic DNA from a wide variety of plant materials and the magnetic particle based DNA extraction protocol can be easily automated using the oKtopure™ automated robot developed by the instrumentation team at LGC Genomics.

oKtopure™ - Automated nucleic extraction platform

LGC Biosearch Technologies

The oKtopure™ is a fully automated nucleic acid extraction instrument that enables a new level of high-throughput processing and significant cost savings. This platform combines standardised high-throughput DNA or RNA extraction from a variety of sample types with the proprietary sbeadex™ magentic bead based extraction chemistry. The oKtopure is part of our on-going programme to provide customers with high productivity solutions, first developed and optimised for use within our service labs, where every day we deliver Extraction, Sequencing and Genotyping services. Key to the delivery of consistently high quality and high yield DNA preparations from the oKtopure is its ability to bind and hold the magnetic beads, and hence the DNA in place whilst transferring the buffers required for washing, binding and elution steps. The final aqueous wash used in the sbeadex extraction protocols enables the delivery of nucleic preparations ideal for downstream applications such as SNP genotyping, NGS and Sanger sequencing. Features: High throughput - capable of processing eight 96-well plates (768 samples) in approximately one-and-a-half hours Robust tip washing system reduces consumable costs by 50% or more Preloaded methods for different sample types – fully customizable Extraction expertise and support from scientists who use the instrument daily and have been carrying out high throughput extractions since 1996!

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Extracting Genomic DNA from Plant Material with the sbeadex® Plant Kit from LGC Genomics