Droplet Digital™ PCR: Multiplex Detection of KRAS Mutations in Formalin-Fixed, Paraffin-Embedded Colorectal Cancer Samples

KRAS is mutated in approximately 40% of colorectal cancers. To optimize therapy strategies for personalized care, it is critical to rapidly screen patient samples for the presence of multiple KRAS mutations. This application note demonstrates a multiplexing strategy which has been developed to screen seven actionable KRAS mutations in colorectal cancer samples using digital PCR. This sensitive and inexpensive method reduces the risk of contamination and can be easily implemented for rapid, routine screening of cancer samples.