Application Note: Discovering Potential Diabetic Lipid Biomarkers Using HRAM LC-MS-MS Approach on a Hybrid Quadrupole – High Field Orbitrap Mass Spectrometer
3 August 2016

This poster demonstrates that a HRAM LC MS-MS approach on a Thermo Scientific™ Q Exactive™ HF hybrid quadrupole high field Orbitrap mass spectrometer enables rapid putative biomarker discovery through lipidomics profiling experiments. Lipids play a key role in cell, tissue and organ physiology with diseases such as diabetes which involve disruption of their metabolic enzymes and pathways. Identification of unique lipid biomarkers to distinguish healthy humans compared to those with a disease can have an impact on the early detection of diseases and personalized medicine. Combined with LipidSearch software, the Orbitrap data allowed simultaneous lipid identification and quantitation in the same experiment.