Application Note: A Sensitive, Specific, Accurate, and Fast LC/MS/MS Method for Measurement of Nicotine, Cotinine, 3-Hydroxycotinine, Nornicotine and Anabasine in Human Urine
15 April 2015

This application note illustrates a method simultaneous analysis of nicotine, two of its major metabolites, cotinine and 3-hydroxycotinine, as well as nornicotine and anabasine from human urine samples. The method described uses Strata™-X-C solid phase extraction (SPE) cartridges for sample clean-up and concentration, followed by fast (<6 min) LC/MS/MS analysis using a Gemini® NX-C18 column.