Application Note: A Rapid iMethod™ Test for the Analysis of Fluoroquinolone Antibiotics in Food
10 June 2014

Fluoroquinolones are broad-spectrum antibiotics used in both human and veterinary medicine. The presence of
fluoroquinolone residue in meat has been implicated in the development of resistant pathogens. Because of these concerns, the FDA has banned the extra-label use of these products in food producing animals.

The method included is for the routine analysis of 20 fluoroquinolones and quinolones and their metabolites in animal
tissue at or below 25 ppb. The method used three MRM transitions per compound to provide an extra degree of confirmation.