Application Note: A Novel Strategy to Screen and Profile Steviol Glycosides of Natural Sweeteners in Food Using ionKey/MS Ion Mobility Mass Spectrometry
25 September 2015
Stevia rebaudiana Bertoni is a perennial shrub of the Asteraceae (Compositae) family which is of great economic value due to the high content of natural, dietetically valuable sweeteners in its leaves. The Joint FAO/WHO Expert Committee on Food Additives (JECFA) has established regulations for steviol glycosides demanding a purity level of at least 95% of the seven chemically defined steviol glycosides. Complete residue analysis of steviol glycosides in food, is a challenge due to nature of the task i.e. the detection of low steviol glycoside isomer concentrations in complex food commodities, where generic extraction procedures have been used. This application note illustrates the selectivity of collision cross section (CCS) measurements used in combination with other recent MS technology enhancements for profiling complex mixtures.