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A Fast and Effective Approach to Analyzing Hormones in Drinking Water Using SPE and LC/MS/MS

19 Apr 2015

This application note illustrates a method for steroid hormone analysis from drinking water using an optimized LC/MS/MS method. Analysis time is reduced from 50 minutes down to about 15 minutes while maintaining excellent linearity (0.99 R2 value or greater for each compound) and low minimum reporting level MRLs. The method presented uses a Gemini® 3 μm NX-C18 HPLC column, which delivers stability at pH 1-12. This improved method should greatly increase lab efficiency and productivity.

Gemini pH Flexible LC Columns

Phenomenex Inc

Setting the Standard for pH Method DevelopmentRugged reversed phase HPLC columns that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations. Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity Expect longer column lifetime with patented TWIN-NX™ technology Ideal for analytical and preparative separations of basic and acidic compounds Who needs Phenomenex's Gemini HPLC columns? Method Developers: Extreme pH method flexibility Reproducible performance Purification Chemists: Purify bases at high pH Increased loading Increased retention Increased resolution Lab Managers: Extended column lifetime Consistent results.

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A Fast and Effective Approach to Analyzing Hormones in Drinking Water Using SPE and LC/MS/MS