Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital™ PCR

Genome editing has become a widely used tool and allows specific modification of almost any cell's genome. However, the often low frequency of gene edits, in particular for some primary or induced pluripotent stem cell types, hinders facile and reliable detection by gel- sequencing-based methods, or requires laborious clonal isolation or edited cells. This application note demonstrates an ultra-sensitive, cost-efficient Droplet Digital PCR-based method for the detection of HDR and NHEJ allels generated using the CRISPR/Cas9 and TALEN genome editing systems.