Application Note: The development of a sensitive and reliable qRT-PCR-based SARS-CoV-2 wastewater analysis protocol
18 October 2021

Wastewater testing provides valuable insight on community-level data for various analytes, such as microbes that are associated with infectious diseases. In this application note, Agilent Technologies describes how to use quantitative reverse transcriptase PCR (qRT-PCR) to determine SARS-CoV-2 viral RNA levels in raw wastewater. This enables the identification of trends in viral load within communities, potentially providing predictive models for the community and healthcare institutions.