ResourceSpectroscopy

PLATEMASTER® and EXTRACTMAN®: Rapid Enrichment and Clonal Selection of CRISPR Modified Cells

1 Oct 2017

CRISPR is changing the face of how scientists are exploring new drug targets and uncovering how diseases work at the genetic level. However, as with any biological system, CRISPR is not 100% efficient, requiring screening to identify and isolate the cell or strain carrying the desired modification. EXTRACTMAN® enables rapid isolation of proteins that bind to an antibody-coated paramagnetic particle, and is a fast and efficient tool that can be used to enrich populations of CRISPR modified cells when an antigen based selection marker is used.

Gilson PLATEMASTER 20uL

Gilson, Inc.

PLATEMASTER® is an easy-to-use, accurate solution for high-throughput manual pipetting of 96- and 384-well microplates. Two models are available: PLATEMASTER P20 for pipetting volumes from 0.5−20 µL and PLATEMASTER P220 for pipetting volumes from 2−220 µL.  Increase the Productivity of Your Workflows The PLATEMASTER’s 96-channel design greatly reduces the number of pipetting steps necessary to fill a microplate when compared to using regular manual multichannel pipettes. When using PLATEMASTER, the time it takes to fill 96-well plates is significantly reduced to approximately 10-20 seconds or less, and 384-wells can typically be filled in less than a minute using only four pipetting steps. Increased Accuracy, Precision, and Reproducibility Using PLATEMASTER for higher throughput applications reduces the risk of human error. In a single motion, accuracy and precision levels can equal that of an 8- or 12-channel pipettes, while avoiding the risk of skipping or repeating wells. Additionally, all enzymatic reactions and cell-based applications across all wells can be started and stopped at the same time. From 0.5 µL to 220 µL pipetting volumes, expect improved well-to-well and plate-to-plate reproducibility. Versatile and Easy to Use PLATEMASTER is as intuitive as a PIPETMAN® and does not require programming, long training, or power for use. Through a variety of accessories, such as adapters for loading 384-well microplates, reagent reservoirs, or sample heater blocks, PLATEMASTER can be fully adapted to a variety of application needs. Its compact and portable design easily integrates into the lab, allowing it to fit on most benchtops, under hoods, or in cold rooms.

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