Peptide quantification in plasma using the Agilent 6495 Triple Quadrupole LC/MS coupled with the Agilent 1290 Infinity II LC System
30 Sept 2020In translational research, increased throughput as well as increased multiplexing mass spectrometry using MRM-based LC/MS methods have become popular for targeted, bottom-up quantification of protein biomarkers. Researchers often target signature peptides from a limited number of proteins in large cohorts and monitor their expression levels during a specified time period, often leading to hundreds or thousands of biological samples. In such large-volume studies, high-throughput, robustness, and reproducibility are essential when deploying LC/MS methods. This application note demonstrates the quantitative analytical sensitivity of 99 signature peptides from 75 protein biomarkers in plasma using the 6495 triple quadrupole LC/MS system and AJS ionization source.
For Research Use Only. Not for use in diagnostic procedures.
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Agilent 1290 Infinity II LC System
Agilent TechnologiesThe 1290 Infinity II LC System is an ultra-high performance LC that has market-leading sample capacity and is designed for the lowest dispersion for the most challenging ultra-HPLC experiments. The 1290 Infinity II LC system is designed to be both a HPLC and high-end UHPLC system in a single flow path, giving you robust and excellent performance no matter what application you are running while seamlessly integrating into your existing laboratory environment.