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Application Note: Native SEC/MS analysis of mAbs on an entirely biocompatible flow path
23 March 2023

Size exclusion chromatography (SEC) of recombinant therapeutic proteins, such as monoclonal antibodies (mAbs), is an essential analytical tool to detect the presence of size variants, which may have adverse effects on safety and efficacy. SEC is commonly carried out using nonvolatile phosphate buffers, which makes the separation incompatible with mass spectrometry (MS). For this reason, a desalting step (online or offline) is required for peak identification. In this application note, learn more about the potential of the combination of a polyether ether ketone (PEEK)-lined Agilent AdvanceBio SEC column with the Agilent 1290 Infinity II Bio LC System for native SEC/MS analysis of mAbs.