Application Note: mRNA cap purity analysis on a Biozen oligo column
23 May 2023

In order to preserve mRNA from 5’ nuclease degradation, a variety of CAPping molecules have been created to preserve the biological stability of the precious mRNA sequence. In this application note from Phenomenex, discover how mRNA oligonucleotide strands of 1250 nucleotides were enzymatically digested with Rnase H and injected into a Biozen™ 2.6 µm Oligo column. Phenomenex has taken a liquid Chromatography (LC) approach paired with high-resolution mass spectrometry (HRMS) to distinguish a pool of multiple caps and their relative integrity.