Application Note: Lysing Micro-Organisms with the Vk05 15 Ml Precellys® Lysing Kit
13 June 2017
Microbes such as yeast or bacteria are difficult to lyse due to their thick cell wall, which makes nucleic acid extraction difficult. Gram positive bacteria for example, have a cell envelope comprised of the plasma membrane and a thick cell wall containing peptidoglycans. This application note demonstrates how the Precellys® homogenizers offer an alternative and more effective lysing solution for DNA and RNA extraction to be used for molecular biology research, such as sequencing, PCR or cloning.