Identification of insulin oligomeric states using SEC-MALS

To ensure patient safety, it is important to understand the molecular properties of the insulin being taken by patients, for example, which oligomeric species are present. This application note from Wyatt Technology discusses how the combination of column chromatography and multi-angle light scattering (MALS) can be applied as a powerful tool to identify monomers, dimers, hexamers and higher aggregates of insulin.