ResourceSeparations

Identification and Quantitative Analysis of Egg Allergen Peptides Using Data Independent Ion Mobility Mass Spectrometry

23 Nov 2017

Egg based proteins are one of the most frequent causes of adverse reactions in food. Since many processed foods contain egg as a raw ingredient, the ability to assess changes in protein structure and detection through the manufacturing cycle is important. Although only egg proteins were the focus of this work, other proteins relating to other allergens were observed, providing a potential means for multi-allergen detection.

ACQUITY UPLC M-Class System

Waters

The analysis you’ve always desired for nano- to microscale separations The ACQUITY UPLC M-Class System delivers the most robust, reliable, and highest quality nano- to microscale UPLC separations to every laboratory performing LC/MS analyses. With unprecedented usability, the ACQUITY UPLC M-Class System enables laboratories to succeed in analyzing compounds that are severely limited in amount or availability, even amid a complex matrix over several orders of magnitude. Confidently achieve your laboratory goals with high-efficiency ACQUITY UPLC M-Class Columns ranging from 75 µm to 1 mm I.D. by using complementary mass spectrometry to support our full range of column I.D.’s, or run 300 µm to 1 mm I.D. columns with optical detection.The system also offers a range of flexible configurations for HDX technology, UV detection, and 2D analysis.ACQUITY UPLC M-Class System Features: Highest sensitivity - UPLC separations yield increased analyte concentration for higher UV and MS signal responses Maximum chromatographic resolution - New 15k psi-capable ACQUITY UPLC M-Class Columns deliver sub-2-µm particle performance Faster nano- and microscale separations - Increased operating pressures yield maximum flexibility for higher throughput analyses Higher resolution with longer columns - Increased pressure enables more separation power Confidence in sample analysis - New non-reactive wetted surfaces throughout the fluidic path minimize absorptive losses Increased solvent compataibility - Improved range of solvent blends precisely metered for gradient fidelity Increased sample capacity - Sample organizer compatibility allows you to store a wide variety of plates and vials in the system for longer, unattended operation UV compatibility - UV flow cells compatible with 300 µm to 1.0 mm I.D. columns to augment your UPLC/MS analysis Scalability - With flow rates from 200 nl/min to 100 µL/min, achieve the best chromatographic performance, from 75 µm to 1.0 mm I.D. columns covering a 170-fold range of column sizes Increased flexiblity - More value configurations enable a variety of applications, icnreased throughput, and maximized peak capacity

(5)

Links

Tags

Identification and Quantitative Analysis of Egg Allergen Peptides Using Data Independent Ion Mobility Mass Spectrometry