Application Note: Hot MolTaq 16S/18S – DNA-Free Hot Start Taq for Bacterial and Fungal PCR Detection at Low Loads
8 November 2018
There is a high demand for ultra-pure PCR reagents, in particular heat stable DNA polymerases, for sensitive assaying of bacteria and fungi at low loads. Applications include sterility testing of health care and pharma products, monitoring of laboratory animals for infections, microbial contamination of food and feed, infection of crops, diagnosis of infectious diseases and other. Quality measures for polymerases like Taq DNA polymerase demand high amplification activity, high target specificity by hot start characters and at the same time very low contamination by microbial DNA. Here, a new Taq DNA polymerase, Hot MolTaq 16S/18S, is presented that combines all of the mentioned quality characters and is explicitly useful for bacterial and fungal DNA screening purposes.