Ensuring qPCR data reliability: Controlling for contamination

To realize the potential of single molecule detection using PCR, two critically important factors must be optimized: the specificity of the individual amplification oligonucleotides to the target sequence, and ensuring that the system is free from any material that could serve as a template for the generation of a false positive signal.

In this application note, LGC, Biosearch Technologies presents contamination cause and mitigation strategies and outlines ways to ensure the reliability of qPCR data.