Automating library prep for NGS-based genetic testing

20 Feb 2020

Many common bottlenecks and quality problems in the next-generation sequencing (NGS) workflow can be traced to the laborious and time-consuming task of preparing sequencing-ready libraries. In this guide, Tecan explains how automating library prep can help improve confidence in the NGS pipeline, and what to consider when choosing automation solutions.

Fluent® Laboratory Automation Workstation for Genomic Workflows

Tecan

Fluent’s groundbreaking design delivers more capacity and productivity for genomic workflows. This innovative workstation offers superior precision, higher throughput and longer walkaway times, allowing scientists to get more done in less time, with greater confidence. The Fluent Laboratory Automation Workstation for genomic workflows offers high precision liquid handling – down to 200 nl – for accurate quantification set-up and normalization of DNA, as well as PCR/qPCR set-up and NGS library preparation. It’s freely configurable architecture allows integration of devices for molecular biology protocols, and can easily be adapted to additional workflows. Whether you want to increase throughput or reduce variability for nucleic acid purification, quantification and normalization or reaction set-up, Fluent provides greater throughput and more consistent results.   The platform’s intuitive FluentControl™ software allows protocols to be written for virtually any genomics application, with dedicated commands for creating standard curves, DNA/RNA normalization, etc. An Active Stop and Resume function permits instant access to the workdeck during a run – allowing set-up errors or omissions to be corrected simply by opening the door – while advanced Method Recovery options further enhance process security. Features: Choice between liquid and air displacement technologies Minimizes risk of errors with smaller sample volumes Built in touchscreen interface Enhanced process security FluentControl Software Integration options  

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Automating library prep for NGS-based genetic testing