ResourceSeparations

Analysis of sugar alcohols and allulose using an Arc HPLC System with refractive index detection

22 Jun 2022

In this application note, an analytical method was developed to detect and quantify sugar alcohols and allulose in beverages and other low-calorie products. Arc HPLC-RI was combined with an Atlantis Premier BEH Z-HILIC analytical column, which provided an increase in polar analyte retention and offered a different selectivity when compared to other hydrophilic interaction liquid chromatography (HILIC) chemistries.

Atlantis Premier BEH Z-HILIC Column

Waters

Atlantis Premier BEH Z-HILIC Columns provide an increase in polar analyte retention and offer a different selectivity when compared to other HILIC chemistries, both powerful attributes when performing HILIC methods development.  Built using rugged ethylene bridged hybrid particles, these columns are pH stable from pH 2-10; the MaxPeak High Performance Surface hardware technology significantly reduces unwanted analyte/surface interactions that can lead to poor peak shape and losses in signal intensity.

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Arc HPLC System

Waters

Count on the Waters Arc™ HPLC System for high-efficiency separations and quality data to help meet your regulatory requirements with confidence.  The Arc HPLC system allows you to easily replicate and improve the performance of existing LC methods without compromising data quality.

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Analysis of sugar alcohols and allulose using an Arc HPLC System with refractive index detection