Application Note: Advances in CRISPR-Cas9 gene editing and screening technology
24 January 2020

CRISPR-based gene editing enables researchers to make site-specific DNA edits resulting in permanent functional changes to the genome.

Originally discovered in bacteria as a defense mechanism against viral attack, the CRISPR-Cas system has since been adapted for a variety of genetic engineering applications. In this application eBook, we identify a number of applications for gene editing and functional genomics screening using Horizon Discovery’s Edit-R™ CRISPR genome engineering platform and cover:

  • Gene knockouts
  • Knock-in with homology-directed repair (HDR)
  • DNA-free CRISPR-Cas9 gene editing
  • Transfection optimization
  • Editing stem cells and transgenic animals
  • Functional genomic screening
  • Transient activation or inhibition of endogenous genes (CRISPRa/CRISPRi)

Related Application Notes