Application Note: 12-hour dissolution of an Extra Strength Excedrin brand analgesic in 200ml of media with the Axcend Focus LC
8 February 2023

The Axcend Focus LC® High-Performance Liquid Chromatography system (HPLC) delivers repeatable analyses of Active Pharmaceutical Ingredient (API) dissolution. In this application note, an Extra Strength Excedrin® analgesic tablet was dissolved, without stirring, in a beaker containing 200 mL of media [97% water; 3% acetonitrile (ACN); and <0.1% trifluroacetic acid (TFA)]. With samples acquired approximately every 12 minutes, the three APIs (acetaminophen, aspirin, and caffeine) were well separated at 4.5 min/run with an average retention time of 2.75 min, +/- 0.05 min RSD (relative standard deviation). Additionally, only 513 µL (0.51 mL) of organic and aqueous solvents were consumed during the 12-hour experiment, resulting in dramatically lower costs for solvents and waste disposal.