Application Note: The effect of column hardware on the analysis of synthetic oligonucleotides by LC-MS
15 September 2023

In this application note from Phenomenex, explore the importance of therapeutic oligonucleotides in pharmaceuticals, which require specialized ion-pair reversed-phase chromatography (IP-RPLC). Traditional hardware can lead to non-specific interactions, affecting analyte recovery and sensitivity. BioTi, a bio-inert titanium tube, improves chromatography, boosts sensitivity, and resolves issues with double-stranded RNA and single-stranded nucleic acids like nusinersen. In summary, BioTi hardware enhances oligonucleotide analysis for more consistent results.