Application Note: Sensitive detection of low-level byproducts in a small molecule reaction by the Agilent 1260 Infinity II Prime online LC/MS system
18 January 2023

In the production process of modern small-molecule pharmaceuticals, it is of crucial importance not only to control the educts and products through the course of the reaction, but also the possibly occurring impurities. Such impurities can pollute the valuable active pharmaceutical ingredient (API). This would make it necessary to introduce additional costly purification steps into the production. This application note demonstrates the online monitoring of a chemical reaction with an occurring byproduct by means of the Agilent 1260 Infinity II Prime Online LC/MS system, including a mass spectrometer, the Agilent LC/MSD iQ single quadrupole. The inclusion of the MSD iQ offers additional sensitivity and selectivity for the identification of low-level impurities for more reliable control of chemical reactions.