Application Note: A Novel Method for Generating Single Intestinal Organoids for High-Throughput Screening
25 March 2019
In this application note, the formation of gastrointestinal organoids derived from human induced pluripotent stem cells (hiPSC) is demonstrated — while using Corning® 96-well spheroid microplates, in combination with Corning Matrigel® matrix. This results in a single intestinal organoid in each well. Differentiation into definitive endoderm and intestinal lineage is confirmed by flow cytometry and immunostaining methods.