Application Note: A novel chromogenic in situ hybridization assay
23 November 2021
In situ hybridization is the gold standard to localize nucleic acid targets within cells and tissues with spatial information. The RNAscope in situ hybridization technology has been a leader in the field of spatial genomics providing single-molecule detection of RNA at single-cell and subcellular resolution. The technology has evolved to encompass detection of specialized RNA targets such as splice variants, highly-homologous sequences, and point mutations using the BaseScope™ assays, and miRNA, siRNAs, and anti-sense oligo detection using the miRNAscope™ assays.