MMP-2 Antibody (2C1) - (Pro and Active)
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB200-113
- Host
- Mouse
- Reactivity
- Guinea Pig, Human, Mouse
- Applications
- ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Immunoprecipitation, Western Blotting
![Western Blot: MMP2 Antibody (2C1) [NB200-113] - Samples: Lane 1 - Human Recombinant MMP-2. Lane 2 - Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - NB 200-113, secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/2ac9c25c26e284305fe6d870ee9d84c5beac9b6e-187x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody [NB110-40878] - ADFP antibody was tested in mouse liver using DAB with hematoxylin counterstain.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/e7d8668d7c0d0dc2ee8bc0003f91939e8b6c230e-400x334.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: PGP9.5 / UCHL-1 Antibody [NB300-676] - in Neuro2a cells with FITC (green). Nuclei were counterstained with Dapi (blue).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/d935e576e6cb720b649ce2d8d11fc1f85cefe9dc-400x296.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP1-92693. Staining of mouse brain cells - Trypsin-EDTA digestion, 2% PFA fixation, primary used at 1:10 dilution/ 1 hour RT incubation (Image provided by Dr Xingrao Ke from Dept. of Pediatrics at Medical College of Wisconsin; see the product review on the Alexa Fluor 700 version of this antibody for methodology details).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/878f436365b591ffa7e4e00dc5811c2937ef315d-400x284.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: XRCC3 Antibody (10F1/6) [NB100-180] - XRCC3 detected in HEK293 lysates using a 1:1,000 dilution of Protein A purified NB100-180. Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts Medical School.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/192307132ff74f86a7102f80369ed6cfa91cc00d-217x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: AKR1B10 Antibody [NBP1-44998] - AKR1B10 expression in 1) aldose reductase (AR) protein, 2) AKR1B10 protein, 3) 293T cells expressing AR and 4) HCT-8 cells expressing AKR1B10. Image courtesy of the laboratory of Dr. Deliang Cao at Southern Illinois University School of Medicine.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/02d36c54cbd39b8985656ede8d8e2d827a879970-400x253.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry: ATF3 Antibody [NBP1-02935] - Analysis of ATF3 in human prostate using Fast Red with hematoxylin counterstain.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/bb919df76f3187ac743692d112ca34ed712a4040-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry: ATP13A2 Antibody [NB110-41486] - Staining of neurons and neuropils using a concentration of 10ug/ml. Human brain, 40X.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8399e10d54333dc23f288479c3df7d39004a4948-400x313.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Arrestin beta 2 Antibody [NBP2-24569] - analysis of ARRB2 in HUVEC cell lysate in the 1) absence and 2) presence of immunizing peptide using ARRB2 antibody at 0.25 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/21275185820b10c472ee76e2e819ab179f979f03-294x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: active/cleaved Caspase 7 Antibody [NB100-56115] - analysis of Caspase-7. Recombinant catalytically active Caspase-7 was western blotted with anti-Active/Cleaved Caspase-7 . The antisera detected both the large and small subunits of active/cleaved Caspase-7.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/e0b9dd3ca6e77c0a9f6bc047a328767982ed6c56-400x339.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Cas9 Antibody (7A9-3A3) [NBP2-36440] - Hela cells were transiently transfected with an N-terminally Flag-tagged S. pyogenes Cas9 expression vector. The cells were stained with the Cas9 antibody followed by anti mouse-AF488 coupled secondary antibody. Nuclei were counter-stained with Hoechst 33342.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/1e8c126c112ed883d1abecd61a4ab2977dfc600f-400x397.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast adenocarcinoma using CLA antibody (clone HECA-452) at 5 ug/ml concentration. The antibody generated a membrane-cytoplasmic staining in the cancerous cells of breast adenocarcinoma. Note:- Besides lymphocytes, CLA has been shown to be present on cells of normal endometrium as well as breast cancer tissues (PMID 19625313 and 22970241).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/a8403f9b1fa09d434101ef8f1f6e7b578ae8d675-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/3d974358df3ef25eee3620b309ded8017a8d32d4-400x150.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: KCNH1 Antibody [NBP1-42816] - Analysis of KCNH1 expression in rat brain lysate.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/eacdbc680bb61a13e6c632fd36bc871fd9b3663c-337x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in trapezoid body in mouse brain using NB300-189 and anti-rabbit Cy3 conjugated secondary antibodies (red color). DAPI was used to counterstain cell nuclei.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/dfbb7134321084a9fcc82701d1413f630719b507-400x372.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Occludin Antibody [NBP1-87402] - Staining of human cell line A-431 shows positivity in plasma membrane & cell junctions.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/eb616852d367a197f970a559b39215ded690ffd0-400x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: PABP Antibody (10E10) [NB120-6125] - PABP antibody was tested in HeLa cells at a 1:50 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/31426e72877ed39f9425edc5ce76f4ac71e5d287-400x302.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - PIEZO1 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/6196cd8828c49ccf5241822bb9142faa8341a11f-400x294.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Sonic Hedgehog Antibody [NBP2-22139] - Sonic Hedgehog antibody was tested in SH-SY5Y cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/5d117b4a38b8508f0ebd3cbeee0b04d25b6f6a38-400x287.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: Synuclein-alpha [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [NBP1-26380] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using Synuclein-alpha [Nitrate Tyr125, Nitrate Tyr133] antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/08f26b1abff930a427b2c3ca9334d51afb0504b7-400x188.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: VLDL Receptor Antibody (6A6) [NBP1-78162] - VLDL Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/09bee2d6b8fe18ea81ec70b7b9bab076c08229e9-400x291.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Olig2 Antibody [NBP1-28667] - Neonatal rat brain lysate showing specific immunolabeling of the ~ 32k Olig2 protein.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/f91fff8207a8cb4f1452eb7ed90cf4d9031e133e-400x291.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry: FoxP3 Antibody [NB600-242] - Staining of CD25-sorted (Treg) human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0717317a8c4ae184f1b645e5adedce8652a071fc-400x151.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: PCSK9 Antibody [NB300-959] - IHC (P) staining of formalin fixed, paraffin embedded human kidney using the PCSK9 antibody at 4 ug/ml. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/438b40ad3794e05dfee5218d95b9b4e8fee460f2-400x305.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: FoxC1 Antibody [NB100-1268] - Staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7467d0ea609aab75384691234d3813bbec46ef0e-400x299.jpg?w=3840&q=75&fit=clip&auto=format)