RelA/NFkB p65 Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB100-2176
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Applications
- Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Immunoprecipitation, Western Blotting
![Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody [NB100-2176] - Staining of formalin-fixed, paraffin-embedded human DLBCL showing nuclear expression of NFkB p65 in the tumor cells.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b6e4d100f7e42da8bc29b82792a856bbe3c25ae6-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Factor Inhibiting HIF-1 Antibody (162c) [NBP1-30333] - FIH (162C) antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/cfd99800aa3d2176c10bc757acffb50a84283c46-400x296.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: MAP2 Antibody (5H11) [NBP1-92711] - Western blot of whole rat brain lysate probed with NBP1-92711 antibody to MAP2. Note that the strong single band running at about 280 kDa corresponds to MAP2.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8cfc4889f2f6a4b1662c386886bbfaa72e2b9ded-96x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Mitofusin-1 Antibody [NBP1-51841] - Mitofusin-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b7473dc565aca90547fdd9b2aa6265927d1e3da5-400x390.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: NFkB p65 Antibody [NBP1-96139] - WB analysis of NFkB p65 in: 1. NIH 3T3 cell lysate and 2. Cos7 cell lysate.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7b86bd7a9d222867257f4cc58746ad096903fef7-178x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: SCP3 Antibody [NB300-230] - Mouse pachytene preparation (red). CDK2 staining, near teleomeres, is also present (green).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/19e455f3ba8a7076433229540435db40e5576673-400x268.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: RANKL Antibody (12A668) [Azide Free] [NB100-56593] - analysis of Trance (RANKL) using Trance antibody. Transfected cell lysate was probed with 2 ug of Trance antibody.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0bc64b06b761cb38096810378ab68c20152c7f4e-230x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: CD3 Antibody (OKT3) [Azide Free] [NBP2-24867] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-24867. Staining of human PBMC. Image from verified customer review.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8f475aae3aee6e6dc1f19b3acc5f306780641a3c-400x387.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in mouse liver lysate.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/ff9fa816dac1c65e37aa31212dc5c5c4ad4eddb0-229x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: LANA2 Antibody (CM-A807) [NB200-167] - Detection of LANA2 in infected lysates (30 ug) using 2 ug/ml of NB 200-167. ECL detection 1.5 minutes. Lane 1: BCBL-1 (KSHV+) Lane 2: BC-1 (KSHV+/EBV+) Lane 3: BCP-1 (KSHV+)](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/53026e4694a7fffee0746919cf116ee573832c6a-226x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-EDG2/LPAR1/LPA1 (NBP1-03363) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/59e63793f32b15a0cbedb0af7ab5f9ba23fffdaf-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: MAT2A Antibody [NB110-94158] - Detection of MAT2A in HepG2 whole cell lysates.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4dc4190371f40d2b42a778aa7d6e95b916c33aaf-258x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Nac1 Antibody (3) [NB110-77345] - Staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/244f87fd57f6e6802cc034a043789a20a23c5292-400x296.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Nogo Antibody [NB100-56681] - analysis of human testis using Nogo antibody at 1:100.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/6bfd6e7296e3b664ed523970779c5402e05c19a7-400x317.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: NTH1 Antibody [NB100-108] - Analysis of NTH1 expression in 1) HeLa, 2) A-431, 3) MCF7 whole cell lysates.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/e5a41def20e8de9a59548b1e7309c565c994ac30-400x379.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody [NBP1-77686] - NeuN antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI(blue) and Dylight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b0111528687bffc7407f77852f379f3dd04da245-400x296.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/03dc4672174aa084369a9c96d55e09dcbcb1cffc-278x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: SSEA-1 Antibody (MC-480) [NB100-1831] - Staining of NTERA-2 cells using NB100-1831 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/9029b9683325aa83dd2b51716f5a2771f7350d8a-400x180.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8b2dcf183f6ddc4e1a07b5a161f37d951e291322-400x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: WNK4 Antibody [NB600-284] - Western Blot Image of anti-WNK4. Whole cell protein from Hek293 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WNK4 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/5c6df731e8833e819b8691eef5346cf609839559-247x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: XRCC3 Antibody [NB100-165] - Detection of XRCC3 (38 kDa) from Hela cell extract using NB100-165 (1:2500). Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/61466104f5d040f67e20e855e25ddbef1a675d3a-112x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Wnt5a Antibody [NBP2-24752] - Analysis of Wnt5A in formalin-fixed, paraffin-embedded human breast tumor using an isotype control (left) and Wnt5A antibody (right) at 10 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4cf1070b6d1f86b702ec2594fb4d324fecaa4bd1-400x202.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: WRN Antibody [NB100-471] - Analysis of WRN in untreated, asynchronous HeLa cells using anti-WRN antibody. The primary antibody was used at a dilution of 1:100, incubated for 1 hour at room temperature in 1XPBS. Image from verified customer review.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/f26182797153e1834c3a32565b6deb1dfa29e703-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: NALP3 Antibody [NBP1-77080] - K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0f22b46c5a66e2e1aceb58b7b4933152cb58abd6-258x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Azide Free version of NB100-56582. Detection of 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/5debfba46f893a4b5034d77840e22b610922dcc6-227x400.jpg?w=3840&q=75&fit=clip&auto=format)