Prolactin R Antibody (U5)
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB300-561
- Host
- Mouse
- Reactivity
- Bovine, Human, Mouse, Pig (Swine)
- Applications
- Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Immunoprecipitation, Western Blotting
![Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561] - Mammary gland tissue from lactating light horse mare (equine) was incubated in anti-prolactin receptor monoclonal mouse antibody at 1:100 for 1 hour at 23C and for 24 hours at 4C. Second antibody was biotinylated goat anti-mouse IgG at 23C for 1 hour. Development using avidin-biotin complex 23C for 1 hour plus 3,3-diaminobenzadine for 15 min and counterstained with haematoxylin.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8e66cbd8fb5e29e1b342ff611a79c5951bfbb013-400x314.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry: LOX Antibody [NB110-59729] - Staining of LOX in mouse spleen.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/55c36f520449f57d8ebbdb84304907a28dd4269d-400x370.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: TLR5 Antibody [NBP2-24827] - Intracellular flow analysis of TLR5 in Balb/c mouse splenocytes using TLR5 antibody at 2 ug/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control this antibody ; red represents TLR5 antibody. Goat anti-rabbit PE this antibody was used as secondary antibody.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/2a5a8d45390659a6e6cfb4578a0be62048f2f4b3-400x329.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: 6-Histidine Epitope Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the HRP conjugate of NB100-64768. Detection of His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/671436ddc534ca6b7cb04fcb2951450d3acf1094-390x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Analysis using the FITC conjugate of NB100-65805. Staining of human peripheral blood lymphocytes.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/a71d6d3d4b85008c4e081ebd7149cd963dd7dd9d-400x393.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Mitofusin 1 antibody was tested in 3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/6e4f1cbeff76876119526045bc993bb6caef93cb-400x301.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Immunostaining in HeLa cells. C1: DAPI staining 2 nuclei, C2: anti-mtTFA stain with Alexa Fluor 488 secondary ab (green), C3: Mitotracker Red staining mitochondria. Image courtesy of Elizabeth Wang.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7be471c4269a1ff0b463ead60ff74cb7acbd4417-400x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: NBR1 Antibody [NBP1-71703] - NBR1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/465d95fe475cf250426b6e67688ed36c9d874227-400x296.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/cb29ab1898fa3593dc13ffd4438df1b1723bcd50-400x301.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Formalin-paraffin colon carcinoma stained with pan Cytokeratin Ab cocktail (AE-1 / AE3).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b889d37535e961e10e1d0cbcf56d6e28850b640c-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/47e0bd61e944c4759694ee0a5195252d8e8bacae-254x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/f183c013ca0f0614eddc41dd1b2f07190a90983d-400x148.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/f6e3fc9f96ae9ead06f3004770da00891640bfb4-235x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: STING/TMEM173/MPYS Antibody [NBP2-24683] - IHC analysis of STING protein in formalin-fixed paraffin-embedded mouse lung tissue section using STING antibody at 1:150 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed using DAB reagent and the nuclei were counterstained with hematoxylin. The antibody generated mainly a cytoplasmic staining in the bronchiolar and alveolar epithelial cells.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/23467021252f9b32c2e302d5324f11143d57e790-326x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: TIN2 Antibody (59B388.1) [NB600-1522] - Analysis of Tin2 using Tin2 monoclonal antibody 59B388.1. Human Daudi (A) and mouse NIH 3T3 lysate probed using Tin2 antibody at 2 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0f63c8082db1d73bed260113fbfab2601aba21dd-235x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Staining of NTERA-2 cells using NB100-730at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0e751819e549e5332bf81876031bf3c9a7a29e1e-400x180.jpg?w=3840&q=75&fit=clip&auto=format)
![Simple Western: USP22 Antibody [NBP1-49644] - Simple Western lane view shows a specific band for USP22 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/bc8955aa58261a6cc4be60f17a4f090c22c0626f-91x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Simple Western: GABA-A R beta 3 Antibody [NB300-199] - Simple Western lane view shows a specific band for GABA-A Receptor Beta 3 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/fd4e3e2c94a367e02af1f5976c5b40292e9eba6a-91x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: FUS Antibody [NBP1-50623] - Staining of FUS in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8fc84b33add47bebba5bcb4b379028494becf59e-400x295.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP) from 293T cells. Ureb1/Lasu1 was less efficiently immunoprecipitated by another Ureb1/Lasu1 antibody.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7b60858937b711173470b9a78e4ed0cdd15f45f5-400x259.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: LATS2 Antibody [NB200-199] - Detection of Human LATS2 on HeLa whole cell lysate using NB200-199. LATS2 was also immunoprecipitated by another LATS2 antibody.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0012079474e2075868118e10ad011aefc71315aa-400x270.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252] - FFPE section of human colon adenocarcinoma. Affinity purified rabbit anti-RbBP5 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/eb9a54525352c40bca5a1cb234ac4a8d644e74ae-400x301.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: SMC1 [p Ser966] Antibody [NB100-206] - Neocarzinostatin treated asynchronous HeLa cells.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/9e75bff282ac5a8a8cc09116374dad9931112a72-400x220.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: TLR4 Antibody [NBP2-24821] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-24821. Staining of RAW cells were probed using 2 ug of TLR4 antibody (red) and 2 ug of isotype control antibody (20304AF488, green). Novus's TLR cell surface flow kit (10099K) was used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/bb3ad2a375323151410869e0056576ab6d9040c8-400x333.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Inhibin beta A Antibody [NB110-61019] - Analysis of Hela cells.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/01d893640c61f9b78b4fa04128f75c875a57e0da-400x292.jpg?w=3840&q=75&fit=clip&auto=format)