![Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis using the FITC conjugate of NB600-305. Detection of Lane 1 shows 80 ug and lane 2 shows 20 ug loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. Blots were blocked overnight at 4C with blocking buffer for Fluorescent Western blotting conjugated Gt-a-anti-Rabbit IgG was used for detection. Molecular weight estimation was made by comparison to a prestained MW marker (center).in lane M.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/00660f49a63e2f864f02ba3fcc6cb6638eadc74a-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
beta Galactosidase Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB600-305
- Host
- Rabbit
- Reactivity
- Bacteria, Mouse
- Applications
- ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting
![Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - Human peripheral blood cells were stained (2 x 10^6 cells/ml) using the anti-CD63 antibody at a dilution of 1:1000. Signal was detected using a Gt x Ms Dylight 488 Secondary and gated to the monocyte/granulocyte cell populations. Data collected on BD FACS Calibur flow cytometer.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7d59e7f0231068a2d7489aaad0fd884a097fed0a-385x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry: Cre Antibody [NB100-56134] - Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the hippocampus of a Cre transgenic mouse at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. Hematoxylin-eosin counterstain. A1 is a higher magnification of A.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0fa85bda9825423402d8efd8189f7457ad90afb1-400x131.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: HSP60 Antibody [NBP1-77397] - analysis of HSP60 in RM1 cells (murine prostate cancer cell line) using anti-HSP60 antibody. The primary antibody was used at a dilution of 1:100. Image from verified customer review.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/041fcb553d84e658016aa61376e26d86ee6fcb60-327x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/1516299dbf3ece80d2570fb6581e2858722a33d9-151x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Kif2a Antibody [NB500-180] - Kif2a antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/d41eb92adbd8c488f3d25fa1c9e652dbde8fb2b6-400x376.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Analysis using the PerCP conjugate of NB100-77668. Staining of bEND.3 cells with MECA-32 AF488.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/1afa51701d827a06cfc640e2f18fbe4a95c2bd9c-370x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Peripherin Antibody [NB300-137] Detection of the 57 kDa peripherin protein in whole rat brain stem homogenate using NB 300-137 (1:20,000). The faint 48 kDa band is a peripherin molecule.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/1d5b7a175e33ca2001e55c32cca0d5d2623cd394-198x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Senataxin Antibody [NBP1-94712] - WB analysis of Senataxin in: A. HeLa whole cell lysate, B. Beads without antibody IP control and C. IP from HeLa lysate.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/26db2ceb9523ec255beb4c5c55ce40be637ce5e1-400x325.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: SG2NA Antibody (S68) [NB110-74572] - Immunofluorescent staining of NIH3T3 cells using NB110-74572.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7b560eeff8031f20f6c58eeb838d5957cfd2271e-243x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Somatostatin Receptor 2 Antibody [NB300-157] - Staining of paraffin embedded rat tissue.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8a3ad3380ad62b14e66f25ce079f15470b691578-400x314.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56563. Staining of TLR1 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram represents cells alone, green represents isotype controlwas used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7b4309c73bbb5e930ed84e3ace9395c7321637d4-400x320.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: WNVM Antibody [NB100-56743] - analysis of WNV-Envelope protein in bird liver using this antibody at 1 ug/ml, incubated for 30 minutes. A Ventana brand second step antibody conjugated to biotin was applied followed with streptavidin-AKP. The substrate was Fast Red chromagen. The antigen retrieval method used to obtain staining: Ventana Protease 2 for three minutes at room temperature with a Parafilm coverslip. (using an autostainer which keeps residual fluid on the slides during every step, so the final concentration is approximately 2.5 times more dilute). Image and protocol used to generate this image are courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/16f443cb0e2d2ad1f3ed8f432f8b317828b8b6b4-400x387.jpg?w=3840&q=75&fit=clip&auto=format)
![Chromatin Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5 % SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade BRCA1 diluted by 1:500](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8fde26198b591ca3c67c654ecb2ba2eb5a69bb9b-210x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus of Human testis tissue (right) compared with a negative control in the absence of primary antibody (left).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/711dbb219da640fe1b16dc1c0da1b89a907f480e-400x198.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with human brain, hippocampus.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/81ce310964eff5369997b4a7e51f01a469ac9fb9-400x316.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235] - IF analysis of BrdU in Proteinase K pretreated mouse lacrimal gland. Image courtesy of anonymous customer review.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/c39c8cb92555c46263206810d8971b9ec12b497b-400x302.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-103] - IF Confocal analysis of 3T3 cells using Calreticulin antibody (NB600-103, 1:20). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/e8668482786b90d24e98fcf20f46de15f1a0d92e-400x385.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: PGP9.5 / UCHL-1 Antibody [NB300-675] - in Neuro2a cells with FITC (green). Nuclei were counterstained with Dapi (blue).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/39c7daf67338011463251feb8d5c2ecdb34eb0ff-400x296.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Aggrecan Neoepitope Antibody [NB100-74350] - Detection of ACAN in OA cartilage using NB100-74350.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/327e6355724bba04e72d8bb83f1884e705ea4687-400x314.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP2-27398] - BAG3 antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/d6672d228832c36a8000c462a8436e0a5b9930f3-400x307.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/378425220c9cfbb4dac9932fc872d4ada887f120-400x293.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [NB120-6586] - Analysis using the Biotin conjugate of NB120-6586. Staining of Collagen IV alpha 1 (red) and Insulin (green) in mouse pancreas. Image from verified customer review.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/2815bad79fcc5d08d52bf6a3f76a89fb3f3ec2e9-400x307.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - Analysis of Cytokeratin 1 in Caco-2.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/391a74cbe11c84f327f5f89b2ed8bc7bcba99a7b-225x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Dopamine Receptor D1 Antibody (SG2-D1a) [NB110-60017] - Detection of Dopamine Receptor D1b of Sf9 cells transfected with rat D1a using NB110-60017. Lane 1: Sf9-D1a lysate. Lane 2: Sf9-D1b lysate (Please note that these results are similiar to those shown in J. Neuroimmunol. 101:170-187 publication.)](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/c561baa526c3e973f3fd5efb6efcf9fa6b6ae62c-400x316.jpg?w=3840&q=75&fit=clip&auto=format)