Cytokeratin, HMW Antibody (AE3)
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NBP2-29404
- Host
- Mouse
- Reactivity
- Bovine, Dog (Canine), Human, Mouse
- Applications
- ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting
![Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (AE3) [NBP2-29404] - Formalin-fixed, paraffin-embedded skin stained with Cytokeratin, HMW Ab (AE-3).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/2f166383c92235f979c2d277fb730fcf09f4c153-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (AE1) [NBP2-29411] - Formalin-fixed, paraffin-embedded skin stained with Cytokeratin, LMW Ab (AE1)](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/af4c4123071bee70eaa730170dfd764c148aacda-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (SPM115 + SPM116) [NBP2-34347] - Formalin-paraffin colon carcinoma stained with pan Cytokeratin Ab cocktail (SPM115+SPM116).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/730379a59f319c8d7fb33bb55f17b157a7f1252a-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: DAAM2 Antibody [NBP2-24536] - Analysis of human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse skeletal muscle and 4) rat skeletal muscle probed with DAAM2 antibody at 0.5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/061ad47f7562d3efad8f78f5f60b8115ccfd6fc7-280x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Dkk-1 Antibody [NBP2-24701] - Analysis of human Dkk-1 in A549 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) RAW using NBP2-24701 at 1 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/27f598cdca7ad3c312d8157b0dbe887078633463-377x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/a74c76ab15d170992c5664c50ccecddf86ffdb78-207x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Dppa4 Antibody [NBP2-24656] - analysis of DPPA4 in human testis lysate using 1) pre-bleed and 2) this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/d9fc98c580b666e3fc11ad3dd018413311ef89c1-272x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Fast skeletal myosin light chain 1 [p Ser19, p Ser20] Antibody [NB100-1687] - Lane 1: Regulatory Light Chain Non-Phospho recombinant protein. Lane 2: Regulatory Light Chain Phospho recombinant protein. Lane 3: Smooth Muscle Non-Phospho recombinant protein. Lane 4: Smooth Muscle Phospho recombinant protein. Load: 50ug per lane. Primary antibody: Myosin pS19/pS20 primary antibody at 1:1,000 overnight at 4C. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Regulatory Light Chain Phospho. Other band(s): None.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/6c6264476f13239ea05bc50679be9d7ac3b73368-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: FLJ10808 Antibody [NBP2-24587] - analysis of UBA6 in human brain, cortex, using UBA6 antibody at 10 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/eccff8e93f4006b9f6e66030407943f172601561-400x317.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Gastrokine 1 Antibody [NBP2-24766] - Analysis of GKN1 in human stomach tissue lysate using this antibody. 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/53206be04f2ccef45fbd49bc5628fe186eb3be4a-184x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Glutathione Reductase Antibody [NBP2-24940] - analysis of GR (2- Glutathione reductase from S. cerevisiae) in human liver lysate using this antibody. 5 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/f9d47849629b026024d0218e6c3262d7a8be8474-218x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Fragilis Antibody [NB600-1534] - Analysis of Fragilis in mouse ES lysate in the (1) absence and (2) presence of immunizing peptide and in mouse NIH3T3 lysate in the (3) absence and (4) presence of immunizing peptide using Fragilis antibody at 1 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/673bd7d0ce1dce0575c319faf00cddf6b85d823e-314x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: ACPL Antibody [NB100-56433] - Analysis of IL-18 RA/AcPL in (A) Human Spleen, (B) Mouse spleen, and (C) Mouse RAW cell lysate using this antibody.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/6a9e22358e391bd8499f1ac77a25039dfc0957b2-212x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: IRAK2 Antibody [NB100-56447] - Western blot analysis of IRAK-2 in 15 ugs of Jurkat cell lysate using NB100-56447 at 1 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7c053ed20ee76869b873e2f802bbb03d9b3153e4-199x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Laforin Antibody [NBP2-24474] - Formalin-fixed, paraffin-embedded human heart stained with EPM2A antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8be062c24b74d1e86056165e41b42092b70f2989-400x374.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Pin1 Antibody [NBP1-05424] - HeLa cells stained with NBP1-05424 antibody to Pin-1 (1:1000 dilution, green) and monoclonal to fibrillarin, NB300-269 (red). Pin-1 stains the nuclear matrix and, much more faintly, the cytoplasm. The fibrillarin antibody marks nucleoli.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/932415c37ffea883e9f7cd0db5c4306b1e9d9acc-400x316.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: RhoGap12 Antibody [NBP2-23690] - Western Blot Image of anti-RhoGap12. Whole cell protein from A549 (lane 1), MCF7 (lane 2) and HepG2 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:500 dilution of anti-RhoGap12 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/c72fadc028fd3ad0de63aaaea7c46480730497ad-234x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Sumo 3 Antibody [NBP2-24651] - Analysis of SUMO3 (SMT3) in human testis lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse testis lysate using SUMO3 antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/11d735f23eefc028c404f1fa322472856f1c36b6-400x370.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: TCF19 Antibody [NBP2-24586] - analysis of TCF19 in 293 cell lysate in the 1) absence and 2) presence of immunizing peptide using TCF19 antibody at 2 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/32cf9f04b241ff4727649652d27341a630a8df1b-272x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: TROY Antibody [NB120-12126] - Analysis of TROY using NB120-12126 in T98G, a human glioblastoma cell line lysate. A protein band of approximately 46 kD is detected.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b1affc238cee8717ee1603c7e49a7f725136ea8f-238x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: CaMKII alpha [p Thr286] Antibody [NB300-184] - Anaylsis of Rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k beta-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0b1f66a133f650061d0f99ec2c1f26a066faa985-400x328.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Retinoic Acid Receptor alpha Antibody (763) [NB200-322] - Analysis of hippocampal lysate showing specific immunolabeling of the ~48k RAR-a protein.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/9a34fa1ef80e238ca83f05802528886aed68f173-316x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/f84d95206f6ba7a5a919b3ae6db17e8da634a9a1-400x283.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat cortex lysate showing specific immunolabeling of the ~120k TAO2 phosphorylated at Ser181 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubatede (1200 units for 30 min) before being exposed to the Ser181 TAO2 antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/e98ce050d8b1d7e21240aaa86dbded09e81ef6e4-388x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: ABCC5 Antibody [NB100-94881] - (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results with antigen retrieval at pH9.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/23661082c9755534fdca2ecc31054177fab03790-400x296.jpg?w=3840&q=75&fit=clip&auto=format)