Clinical analysis of perfluoroalkyl substances (PFASs) in human serum by LC-MS/MS using Captiva EMR-Lipid cleanup
19 Jan 2022
Efficient sample preparation before LC-MS/MS analysis of perfluoroalkyl substances (PFASs) is an important consideration for research laboratories performing clinical analysis. Phospholipids (PPLs) have been identified as a major cause of matrix effects in the LC-MS/MS analysis of serum samples.
In this webinar, Joseph Homan, Senior Scientist III at NMS labs, will describe serum sample preparation and LC-MS/MS analysis of PFASs using protein precipitation followed by PPL removal using the Agilent Captiva EMR-Lipid cartridges. Captiva EMR-Lipid cartridges produce cleaner extracts, with the removal of over 99% of unwanted PPLs from serum matrix, which reduces matrix ion suppression effects and system contamination. Homan will describe how using the Captiva EMR-Lipid cartridges analysis of PFASs at concentrations as low as 0.05 ng/mL yielded ideal peak shapes with good signal-to-noise (S/N) and linear calibration curves.
Key Learning Objectives
- Understand how Captiva EMR-Lipid cartridges selectively extract matrix lipids without removal of PFASs
- Discover how Captiva EMR-Lipid cartridges allow for a simple extraction that reduces the risk of contamination by PFASs
- Learn how Captiva EMR-Lipid cartridges use universal extraction that allows for analysis of acidic and neutral PFASs without compromise of extraction cleanliness
Who Should Attend
- Clinical research staff
- Food testing teams
- Those involved in environmental sample preparation and analysis
For Research Use Only. Not for use in diagnostic procedures.