Simplified and Enhanced Antibody, Protein and Oligonucleotide Conjugation

Innova Biosciences – Antibody Conjugation Service Bio-conjugates can be challenging to optimise and time consuming to produce: 1. Too much conjugation may result in the target protein becoming inactive and insoluble. 2. Too little conjugation can result in weak signals. 3. Our competitors require milligrams of your precious material and have a 2 week process time. Fast & FAb™ Antibody Conjugation Service – Innova Biosciences take the hassle, time and effort out of making antibody conjugates. Using state of the art conjugation chemistry, Innova Biosciences have the ability to label microgram to milligram amounts of your antibody within 48 hours. Conjugate optimization (Micro-optimizer™) - Innova Biosciences uses its proprietary technologies to enhance the performance of conjugates. By creating an array of trial conjugates (10 formulations per label) at low microgram scale (10µg per formulation) combined with selection in the assay of interest, the best possible conjugate can be attained. PerfectSCALE™ - All conjugates produced using our micro-optimization™ technology can be scaled over many orders of magnitude without the need for extensive re-development of the process. The inherent simplicity of Innova’s technology means that exceptionally high yields are obtained, with minimal batch-to-batch variation, ensuring consistent performance.

Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30 seconds. Simply add your antibody solution to a tube of Lightning-Link™ mix and the label becomes covalently attached to your antibody in a gentle controlled process. Go ‘direct’ Direct antibody labelling greatly simplifies immunodetection techniques. Without the problems of crossover and/or non-specific binding from secondary reagents it is far easier to obtain high quality, pioneering results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry. A reduction in the number of incubation and wash steps also saves you time and money. Alkaline phosphatase (AP) is a hydrolyase enzyme that is frequently used in antibody conjugation for immunoassays. AP has a lower catalytic rate than HRP, but has greater stability and maintains linear reaction kinetics for much longer. PNPP is a popular colorimetric substrate for AP; the product of the reaction, p-nitrophenol, can be read at 405nM. Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate; MUP) may also be employed.

Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30 seconds. Simply add your antibody solution to a tube of Lightning-Link™ mix and the label becomes covalently attached to your antibody in a gentle controlled process. Lightning-Link is the easiest and quickest method available for antibody conjugation.Go ‘direct’ Direct antibodies labelling greatly simplifies immunodetection techniques. Without the problems of crossover and/or non-specific binding from secondary reagents it is far easier to obtain high quality, pioneering results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry. A reduction in the number of incubation and wash steps also saves you time and money. The antibody conjugate: Streptavidin is a 53kDa protein purified from Streptomyces avidinii, which has widespread applications in biotechnology due to its very high affinity to the vitamin biotin. Streptavidin is a tetrameric molecule, composed of 4 13kDa monomers, each of which can bind a molecule of biotin.