29 June 2023
In this video, CURIOSIS examines the anti-mitotic activity of nocodazole against a cancer cell line by monitoring cell division. HeLa cells, transfected with H2B-GFP plasmid, are seeded in a 24-well plate at 4 x 104 cells per well. After allowing the cells to attach overnight, they are incubated with or without 62.5 nM of nocodazole. Real-time imaging is conducted using the Celloger® Mini Plus 4x objective, capturing green fluorescence. Time-lapse images are taken every 15 minutes over a period of 19.5 hours.