epMotion® 5075
The epMotion 5075 is the ideal solution for diverse liquid handling demands. It offers the same outstanding accuracy and precision as epMotion 5070 and 5073. The available options make the 5075 an excellent and flexible device for applications such as NGS library preparation, real-time PCR set-up, magnetic bead-based and filter-based purification, cell-based assays or any routine pipetting tasks.
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Automates precise, high-throughput liquid handling for lab efficiency
Pipetting robot
Easy to use and time saver. Reduce human errors and allow reproducible results.
Review Date: 27 Oct 2025 | Eppendorf
The epMotion is a game-changer for high-throughput lab procedures
Life science
epMotion saves a lot of time—during an eight-hour work shift, a qualified operator can isolate 3 x 96 DNA samples (from plant tissue, using Macherey-Nagel NucleoSpin 96 Plant II chemistry). The device comes with a set of commonly used protocols. It takes some time to optimize and adjust them to your lab’s specific requirements, but once that’s done, all you need to do is press the start button. Pipetting is precise and accurate, even for very small aliquots. The interface is user-friendly and very intuitive. As for technical support, I have never had any issues getting assistance or answers to my questions. We use our epMotion for DNA isolation and purification, preparing PCR mixes, and plate-to-plate sample transfers.
Review Date: 4 Feb 2025 | Eppendorf
Precise and functional
User
Requires time to adapt to the different options but after initial training and use it offers an improvement to manual handling (speed, precision, and reproducibility). A great help for our experiments.
Review Date: 17 Oct 2024 | Eppendorf
The epMotion 5075 is a laboratory instrument with a lot of potential
Liquid Handling, Beads purification
The epmotion 5075t has been integrated in the NKI-AVL, department of Molecular Diagnostics in various lab processes, such as purification by means of beads from amplicon libary's obtained by means of the following applications. Archer Fusionplex kits: Expanded Sarcome Panel, Lungv2 panel and Solid panel. Ampliseq for illumina custom panels and the epMotion 5075t is used for diluting DNA samples and Amplicon Libraries. The epMotion 5075t performs repetitive pipetting tasks that would otherwise be done by an analyst. Standardization with the epMotion 5075t increases the quality, reproducibility and traceability of the analysis and prevents mix-ups of specimens. The epMotion 5075t is a user-friendly robot that, in addition to being low-maintenance, also causes little interference when the robot is used correctly. The deck layout is clear and there are many deck items available with different functionalities. A wide range of plastics, such as eppen and PCR plates, can be used, also from suppliers other than Eppendorf. The analysts can get to work with the epMotion 5075t fairly quickly after instruction. The software is logical and understandable, and new programs are easy and quick to develop and use. The software support is easily accessible and has a short response time to questions that are asked via e-mail. The people are experts and think along with the development of robot programs.
Review Date: 6 Sept 2024 | Eppendorf
Versatile and easy-to-use liquid handling
Automated liquid handling
Ideal to use for any liquid handling applications, such as DNA isolation, dilution series and many others. Programs are easy to prepare with an intuitive interface, allowing you to perform any action you would like. We are very happy with our epMotion and use it often.
Review Date: 6 Sept 2024 | Eppendorf
Awesome robotic instruments for the molecular experiments
Automated system for pipetting and for nucleic acid extraction
This emotion is excellent product to have in your lab when you need to process a lot of samples altogether. Automated system for extraction and pipetting with least error. Great results I got after its use for the PCR run even better with the normal processing through extraction, which is time consuming also. This made my life pretty easy and highly recommended.
Review Date: 14 Dec 2020 | Eppendorf
Liquid Handling
A first time user would need training in programming. It would be nice to find out new ways of carrying out different applications. It gives negligible interday, intraday and interplate variation for my screening assays.
Review Date: 16 Dec 2013 | Eppendorf
LC MS-MS
I am glad we took the decision of going for EP motion 5075 for our LC MSMS application. It has lived up to expectations and has done well to serve our needs. I am looking forward to using it more as we grow in numbers. Application support and service after sale has been very good.
Review Date: 16 Dec 2013 | Eppendorf
Plant Science
We use the epMotion 5075 VAC in different protocols such as PCR, real time PCR, genomic DNA and plasmid extraction and sample dilution. Using the epMotion 5075 VAC and a Fast Real Time equipment it is possible to pipette and run up to six 96-well plates in a working day. For Plant DNA extraction and Plasmid extraction we use kits that had been tested and that Eppendorf provides Plug’n’Prep protocols. In a couple of hours it is possible to extract high quality DNA from 96 samples. You load all reagents and labware, press the start button and while you perform other tasks the epMotion does the bench work for you. The Plug’n’Prep protocols are very easy to use. Creating a new protocol is time-consuming but once you have done it you can save it and use with different number of samples. I would definitely recommend the Eppendorf epMotion 5075 VAC for scientists who need high-throughput and accurate pipetting, sample extraction and purification.
Review Date: 13 Dec 2013 | Eppendorf
Molecular Ecology
I have had this equipment for 2 years. It has worked seamlessly from the start. It is very handy for purification of PCR products for DNA sequencing (using magnetic beads). I would recommend it.
Review Date: 10 Dec 2013 | Eppendorf
With epMotion automated pipetting systems, you will see things in a nepMotion 5075 family overview:
epMotion® 5075l
Don't stress yourself by doing complex pipetting tasks when you can perform fast, accurate and reproducible PCR plate setup with the epMotion 5075l automated liquid handler. The epMotion 5075l offers 15 worktable positions and is well suited for high-throughput preparation of plates, PCR or qPCR setup, DNA/RNA normalization, cherry picking, dilution series, reformatting, and immunoassays. Say goodbye to tedious pipetting steps!
epMotion® 5075t
The epMotion 5075t automated liquid handling system allows you to perform high-throughput NGS library preparation with magnetic beads with speed and efficiency. It is also ideal for automating more complex, time-consuming liquid handling tasks such as DNA/RNA extraction and ELISA. Save time and improve the reproducibility of your results with the epMotion 5075t!
epMotion® 5075v
With integrated vacuum filtration, the epMotion 5075v allows true walk-away automation of filter-based purification or extraction protocols. Increase your lab productivity and free up time for other tasks!
epMotion® 5075vt
With integrated vacuum filtration and a combined mixing and temperature control module in one system, the epMotion 5075vt allows true walk-away automation of both filter- and bead-based, purification or extraction protocols. Do not let complex purifications and extractions tie you to your workbench!
epMotion® 5075t NGS Solution
The epMotion 5075t NGS Solution includes all dispensing tools, accessories, and consumables needed for NGS library preparation of up to 96 samples. It automates the process, providing reproducible and precise results while increasing lab productivity. With maximum kit flexibility, it's ideal for medium- to high-throughput and complex NGS workflows, eliminating the need for labor-intensive manual workflows.
Optimization of the PowerSoil®-htp 96 Well Soil DNA Isolation Kit on epMotion® 5075 VAC
MO BIO Laboratories Inc. developed the unique PowerSoil-htp 96 Well Soil DNA Isolation Kit for isolating genomic DNA from environmental samples while also removing PCR inhibitors. This kit was optimized on the epMotion 5075 VAC automated pipetting system allowing greater pipetting accuracy while offering a more efficient workflow. DNA was extracted from six soil types from multiple sites in central and southwest Michigan using the PowerSoil-htp 96 Well Soil DNA Isolation Kit producing varying yields of DNA sufficient for subsequent testing and amplification of the DNA and showing little evidence of PCR inhibition from common inhibitors such as humic acids. Results demonstrated that DNA yields on the robot were similar to those obtained using individual silica spin filters.
Automated Genomic DNA Purification of Marine Organisms on the epMotion® 5075 VAC from Eppendorf
This application note describes the integration of the MACHEREY‐NAGEL NucleoSpin® 8/96 Tissue kit into the epMotion® 5075 VAC automated pipetting system. The kit can be used for genomic DNA isolation from different marine organisms. Data is presented for corals, red and yellow gorgonians, sponges, crustaceans and echinoderms.
Automated DNA Extraction of Forensic Samples with the DNA IQTM System on the epMotion® 5075 LH
Forensic DNA laboratories are experiencing rapidly growing demand to process large numbers of evidence samples. In an effort to meet the rising needs for DNA analysis, liquid handling workstations are utilized for the automation of the liquid handling tasks. This study demonstrates that the epMotion 5075 LH used in combination with the DNA IQ System (Promega Corporation) is versatile enough to accommodate the whole spectra of samples encountered by a crime laboratory. The performance of the epMotion LH 5075/DNA IQ System with regard to DNA yields and potential cross-contamination for different sample types was evaluated.
A Versatile Method for the Automated Isolation and qPCR Analysis of Mouse Tail Genomic DNA
Isolation of genomic DNA from mouse tails with magnetic beads can be automated with the epMotion pipetting system from Eppendorf. This study describes a semi-automated isolation protocol using of the Invitek InviMag® Tissue DNA Kit and the Invitrogen ChargeSwitch® gDNA Micro Kit in combination with the epMotion 5070 with the Eppendorf MixMate. A fully automated method, developed on the epMotion 5075 LH, for the isolation of gDNA for sensitive applications such as qPCR, is also described.
Automated Purification of RNA from Cells or Tissue
This application note describes the configuration and preparation of the epMotion 5075m/t to automate the NucleoMag 96 RNA kit. The combination of epMotion 5075t/m and the purification kit, allows walk away purification of RNA from up to 96 cell or tissue samples in less than 100 minutes.
Automation of ChIP-Seq Library Preparation for Next Generation Sequencing on the epMotion<sup>®</sup> 5075 TMX
This application note describes the successful automation of Illumina ChIP-Seq library preparation on the Eppendorf epMotion® 5075 TMX, using the KAPA High-Throughput Library Preparation Kit from Kapa Biosystems. ChIP-Seq library preparation can be a challenging procedure to automate because of its low ChIP DNA input. The combined advantages of the Eppendorf epMotion® 5075 TMX and the KAPA High-Throughput Library Preparation Kit offer an industry-leading solution for standard and low-input library preparation, particularly for ChIP-Seq.
From manual to mastered, the smartest way to streamline NGS library prep
Session 1 broadcasting at 9:00 BST | 10:00 CEST
Session 2 broadcasting at 13:00 EDT | 10:00 PDT
Move from manual to automated NGS library prep, without the steep learning curve
Preparing high-quality NGS libraries is essential for successful sequencing. However, as sample numbers grow and pressure increases to deliver consistent results, many labs are turning to automation to boost throughput, reduce hands-on time, and improve reproducibility. If you're relying on manual methods – or just beginning to explore automation – this webinar will show you how easy it can be to make the switch, streamline your workflow, and set your lab up for long-term success.
In this free webinar, join Ebony Courtney and Kirk Mantione for a practical guide to automating gold-standard Illumina library prep workflows on the epMotion® liquid handling system from Eppendorf, a user-friendly solution that saves hands-on time, reduces human error, and delivers reliable results with minimal setup.
Join the session to learn:
- How to optimize Illumina library prep for greater accuracy and reproducibility
- How Eppendorf epMotion simplifies liquid handling for NGS workflows
- How to boost efficiency and reduce error by automating key NGS steps
- What to expect when getting started with automation – no prior experience needed
The webinar will focus on Illumina DNA Prep, Illumina DNA Prep with Enrichment, and Illumina RNA Prep with Enrichment workflows due to their universal application in genomics research.
Who should attend? Genomic researchers, NGS workflow specialists, and clinicians currently using Illumina core library prep kits who are looking to automate for the first time or scale up existing workflows.
A portal to another world – Automated cell engineering in drug discovery
Tuesday, October 21, 2025
To better suit your schedule and time zone, this webinar will be offered in two separate sessions:
Session 1 broadcasting at 09:00 BST | 10:00 CEST
Session 2 broadcasting at 13:00 EDT | 10:00 PDT
Efficient delivery of biomolecules such as small molecules, proteins, peptides, and intracellular assays remains a critical challenge to both cell engineering and drug discovery across scales. While electroporation is widely used, it has high costs, low cell survival rates, and labor-intensive workflows. Mechanoporation offers a promising alternative, though traditional implementations have struggled to achieve scalability.
Join Dr. Armon Sharei, Portal Bio, and Dennis Condy, Eppendorf, as they discuss how the integration of advanced mechanoporation technology with automated liquid handling enables a cost-effective, high-throughput solution for next-generation cell engineering and drug screening workflows, while also maintaining reproducibility across experiments for greater confidence in results.
Key learning objectives:
- Uncover how mechanoporation opens a whole new galaxy of cargo delivery
- Explore how to leverage automation to go beyond current drug screening and cell engineering workflows
- Discover how to maintain reproducibility whilst boosting your sample throughput
Who should attend?
- Discovery and R&D groups across drug discovery and cell therapy
Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.
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